Diagnostic Value of Anti‑HpNAP and Anti‑UreB IgG as Non‑Invasive Serological Markers in Active Helicobacter pylori Infection
DOI:
https://doi.org/10.63950/gshh.2025.1.2.31Keywords:
Helicobacter pylori; HpNAP (Helicobacter pylori neutrophil-activating protein); Urease B subunit (UreB); Serological biomarkers; Stool antigen test, Helicobacter pylori, HpNAP, UreB, Serologic markers, Stool antigen testAbstract
Introduction: Accurate non-invasive markers of active Helicobacter pylori infection are needed. This study measured serum anti-HpNAP and anti-UreB IgG in symptomatic adults and compared levels between stool-antigen-positive and negative patients.
Methods: Cross-sectional study of 50 adults (30 HpSA-positive, 20 HpSA-negative). Anti-HpNAP and anti-UreB IgG were measured by ELISA. Group comparisons used Welch's t-test. Receiver operating characteristic (ROC) analysis and logistic regression were performed.
Results: Mean anti-HpNAP IgG: 112.1 ± 32.1 ng/mL (HpSA-positive) vs 69.9 ± 23.9 ng/mL (HpSA-negative); Welch t = 5.321, df = 47.3, p < 0.0001. Mean anti-UreB IgG: 153.9 ± 50.1 ng/mL vs 102.0 ± 40.8 ng/mL; Welch t = 4.017, df = 46.0, p = 0.0003. AUC (HpNAP) = 0.942; optimal cutoff = 102.56 ng/mL (sensitivity = 0.77, specificity = 0.95). AUC (UreB) = 0.803; optimal cutoff = 112.83 ng/mL (sensitivity = 0.80, specificity = 0.75). Univariable logistic regression OR per 1 ng/mL increase: HpNAP = 1.105 (95% CI 1.041–1.173), UreB = 1.030 (95% CI 1.011–1.050).
Conclusion: Serum anti‑HpNAP and anti‑UreB IgG concentrations are significantly higher in patients with stool‑antigen evidence of active H. pylori infection. These antigen‑specific serologic markers warrant further validation (including ROC analysis and longitudinal assessment) to determine their diagnostic performance and potential clinical roles.
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The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
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